Bioconversion of Corticosterone into Corticosterone-Glucoside by Glucosyltransferase

Bioconversion of Corticosterone into Corticosterone-Glucoside by Glucosyltransferase

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Glucosylation of the 21-hydroxyl group of glucocorticoid changes its solubility into hydrophilicity from hydrophobicity and, as with glucocorticoid glucuronides as a moving object in vivo, it is conceivable that it sophie allport bee curtains exhibits the same behavior.Therefore, glucosylation to the 21-hydroxyl group while maintaining the 11β-hydroxyl group is particularly important, and glucosylation of corticosterone was confirmed by high-resolution mass spectrometry and 1D (1H and 13C) and 2D (COSY, ROESY, HSQC-DEPT and HMBC) NMR.Moreover, the difference in bioactivity between corticosterone and corticosterone 21-glucoside was investigated in vitro.Corticosterone 21-glucoside showed greater neuroprotective effects against H2O2-induced cell death and reactive oxygen species (ROS) compared with whole wheat phyllo dough corticosterone.

These results for the first time demonstrate that bioconversion of corticosterone through the region-selective glucosylation of a novel compound can present structural potential for developing new neuroprotective agents.